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rabbit anti pde5a  (Proteintech)


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    Structured Review

    Proteintech rabbit anti pde5a
    Rabbit Anti Pde5a, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+pde5a/PDE5A+Antibody/bio_rxiv__2024__06__18__599563-259-17-18
    Average 92 stars, based on 22 article reviews
    rabbit anti pde5a - by Bioz Stars, 2026-09
    92/100 stars

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    other:

    Article Title: Exercise training affects calcium ion transport by downregulating the CACNA2D1 protein to reduce hypertension-induced myocardial injury in mice
    Article Snippet: rabbit anti-PDE5A , Proteintech , Cat# No. 22624-1-AP; RRID:AB_2879137.

    Membrane:

    Article Title: Exercise training affects calcium ion transport by downregulating the CACNA2D1 protein to reduce hypertension-induced myocardial injury in mice
    Article Snippet: Protein samples were isolated using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Immobilon, Millipore, Billerica, MA, USA). .. Seal the membrane at room temperature for 1 hour with 5% skim milk in TBST buffer (TBS contains 0.1% Tween-20) and incubate overnight with the following primary antibodies at 4 ° C: rabbit anti-eNOS (1:1000, Proteintech, Wuhan, China); rabbit anti- Natriuretic Peptide Precursor A (NPPA) (1:2000, Proteintech, Wuhan, China); rabbit anti- Brain natriuretic peptide (BNP) (1:500,Proteintech,Wuhan,China); rabbit anti-LAMA2 (1:1000, Proteintech, Wuhan, China), rabbit anti-CACNA2D1 (1:1000, Proteintech, Wuhan, China), rabbit anti-CTNNA3 (1:1000, Proteintech, Wuhan, China); rabbit anti-PKG (1:1000, Proteintech, Wuhan, China); rabbit anti-ACTN2 (1:10000, Proteintech, Wuhan, China); rabbit anti-ITGA7 (1:500, Abcam, Cambridge, UK); rabbit anti-PDE5A (1:2000, Proteintech, Wuhan, China). .. On day 2, TBST was cleaned (three times, 5 min each), followed by appropriate secondary antibody (Anti-Rabbit IgG, 1:5000; Anti-mouse IgG, 1:10000; Proteintech) for 1 h. Immunoreactive proteins were quantified using the NIH ImageJ software. mouse anti-Beta Actin (1:20000, Proteintech, Wuhan, China) was used as an internal control.



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    FIGURE 5 Effects of HS exposure on hypothalamic expression of orthodox aquaporin genes in HWE and LWE chickens. Relative expression of AQP1 (a), AQP2 (b), <t>AQP5</t> (c), AQP4 (d–f), and AQP8 (g–i) gene was determined by qPCR and analyzed by 22−ΔΔCt method (Schmittgen & Livak, 2008) using LWE-TN group as a calibrator. Data are presented as mean ± SD (n = 12 birds/group) and analyzed by two- way ANOVA and Tukey's HSD multiple comparison test. If the G by E interaction is not significant, the main effect (G or E) was analyzed separately by Student's t-test. Asterisk (*) and different letters indicate significant difference at p < 0.05. AQP, aquaporin; E, environment; G, genotype or line; G × E, genotype by environment interaction; HS, heat stress; HWE, high-water efficient; LWE, low-water efficient; TN, thermoneutral.
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    Image Search Results


    FIGURE 5 Effects of HS exposure on hypothalamic expression of orthodox aquaporin genes in HWE and LWE chickens. Relative expression of AQP1 (a), AQP2 (b), AQP5 (c), AQP4 (d–f), and AQP8 (g–i) gene was determined by qPCR and analyzed by 22−ΔΔCt method (Schmittgen & Livak, 2008) using LWE-TN group as a calibrator. Data are presented as mean ± SD (n = 12 birds/group) and analyzed by two- way ANOVA and Tukey's HSD multiple comparison test. If the G by E interaction is not significant, the main effect (G or E) was analyzed separately by Student's t-test. Asterisk (*) and different letters indicate significant difference at p < 0.05. AQP, aquaporin; E, environment; G, genotype or line; G × E, genotype by environment interaction; HS, heat stress; HWE, high-water efficient; LWE, low-water efficient; TN, thermoneutral.

    Journal: Physiological reports

    Article Title: Effect of heat stress on the hypothalamic expression profile of water homeostasis-associated genes in low- and high-water efficient chicken lines.

    doi: 10.14814/phy2.15972

    Figure Lengend Snippet: FIGURE 5 Effects of HS exposure on hypothalamic expression of orthodox aquaporin genes in HWE and LWE chickens. Relative expression of AQP1 (a), AQP2 (b), AQP5 (c), AQP4 (d–f), and AQP8 (g–i) gene was determined by qPCR and analyzed by 22−ΔΔCt method (Schmittgen & Livak, 2008) using LWE-TN group as a calibrator. Data are presented as mean ± SD (n = 12 birds/group) and analyzed by two- way ANOVA and Tukey's HSD multiple comparison test. If the G by E interaction is not significant, the main effect (G or E) was analyzed separately by Student's t-test. Asterisk (*) and different letters indicate significant difference at p < 0.05. AQP, aquaporin; E, environment; G, genotype or line; G × E, genotype by environment interaction; HS, heat stress; HWE, high-water efficient; LWE, low-water efficient; TN, thermoneutral.

    Article Snippet: Primary antibodies used were rabbit antiAQPs (AQP1 # A15030, AQP2 # A16209, AQP3 # A2838, AQP4 # A2887, AQP10 # A2888, ABclona, Woburn, MA), rabbit anti- AQP5 (#orb665771, Biorbyt, Cambridge, UK), and rabbit anti- glyceraldehyde 3- phosphate dehydrogenase (GAPDH, #NB300- 327, Novus Biologicals, nloaded from https://physoc.onlinelibrary.w iley.com /doi/10.14814/phy2.15972 by N guyen T am - T aipei M edical U niversity , W iley O nline L ibrary on [06/08/2024].

    Techniques: Expressing, Comparison

    Journal: Cell Reports Medicine

    Article Title: Phosphodiesterase type 5 inhibitors enhance chemotherapy in preclinical models of esophageal adenocarcinoma by targeting cancer-associated fibroblasts

    doi: 10.1016/j.xcrm.2022.100541

    Figure Lengend Snippet:

    Article Snippet: Rabbit polyclonal anti-PDE5A , Santa Cruz Biotechnology , Cat#sc-32884.

    Techniques: Recombinant, Fluorescence, Gene Expression, Microarray, Mass Spectrometry, Generated, Negative Control, Software